A DNA barcode is a short DNA sequence used as an index or tag to uniquely identify samples in multiplexed experiments such as next-generation sequencing runs.
Extremely high or low GC content can cause biased amplification or sequencing artefacts. Keeping barcodes within a moderate GC window helps maintain more uniform behavior.
Hamming distance is the number of positions at which two sequences differ. A higher minimum distance between barcodes makes them more robust to sequencing errors.
Forbidden motifs are user-specified subsequences that must not appear in any barcode. They are commonly used to avoid restriction enzyme sites, primer binding sites, and adapters.
Yes. On the Home page, once barcodes are generated, you can download them as a CSV file or copy them directly from the table.